The story takes place in New York in 1926, when the resistance of the No-Maj against magicians reached its peak, and the anti-magic organization led by Mary Lou (Samantha Morton) was active. At the same time, the various disasters and accidents that occurred from time to time in the streets and alleys made the existence of the magic world only a line away from being exposed, and the entire Ministry of Magic fell into an unprecedentedly tense atmosphere. At this juncture, the magic zoologist Newt (Eddie Redmayne), who traveled around the world to study magical animals, arrived in New York. The magic suitcase he carried with him was filled with dozens of magical animals he rescued along the way, and his every move was under the surveillance of Tina (Katherine Waterston), a former Auror of the Ministry of Magic. In an accident, No-Maj Jacob (Dan Fogler) mistakenly took Newt's suitcase and released the magical animals hidden in the box. For this reason, Newt, Tina, Jacob and Tina's sister Queenie (Alison Sudol) had to embark on a journey to find magical animals.