The film tells the story of the new generation Spider-Man Miles (voiced by Shameik Moore) and Spider-Gwen (voiced by Hailee Steinfeld) crossing the multiverse to embark on a greater adventure. Faced with the fate of every Spider-Man losing his loved ones, Miles vows to break the curse of fate and find his own heroic path. This decision has caused a great conflict with the Spider-Union led by Spider-Man 2099 (voiced by Oscar Isaac), and a one-versus-hundred Spider-Man civil war is about to begin!